ARG41721
anti-NEK7 antibody
anti-NEK7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes NEK7 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NEK7 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human NEK7. (NP_598001.1) (C-NTLANFRIEKK) |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.11.1; Never in mitosis A-related kinase 7; Serine/threonine-protein kinase Nek7; NimA-related protein kinase 7 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8TDX7 Human Serine/threonine-protein kinase Nek7 |
|---|---|
| Gene Symbol | NEK7 |
| Gene Full Name | NIMA-related kinase 7 |
| Background | NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM, Jul 2002] |
| Function | Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody. [UniProt] |
| Calculated MW | 35 kDa |
| PTM | Phosphorylation at Ser-195 required for its activation. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG41721 anti-NEK7 antibody WB image
Western blot: 35 µg of HeLa cell lysate (in RIPA buffer) stained with ARG41721 anti-NEK7 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.
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ARG41721 anti-NEK7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG41721 anti-NEK7 antibody at 3.75 µg/ml dilution.
