ARG42974
anti-NFIC antibody
anti-NFIC antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NFIC |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NFIC |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to D215-G508 of Human NFIC. |
| Conjugation | Un-conjugated |
| Alternate Names | Nuclear factor 1 C-type; CTF5; CCAAT-box-binding transcription factor; TGGCA-binding protein; Nuclear factor 1/C; NFI; NF1-C; CTF; NF-I/C; NF-I; NFI-C; Nuclear factor I/C |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 56, 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NFIC |
| Gene Full Name | nuclear factor I/C (CCAAT-binding transcription factor) |
| Background | The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011] |
| Function | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 56 kDa |
Images (7) Click the Picture to Zoom In
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ARG42974 anti-NFIC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreatic cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42974 anti-NFIC antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42974 anti-NFIC antibody WB image
Western blot: 50 µg of sample under reducing conditions. HeLa, U2OS, T-47D, PC-3, HepG2, A431, Caco-2, Rat skeletal muscle and Raw264.7 whole cell lysates stained with ARG42974 anti-NFIC antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG42974 anti-NFIC antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42974 anti-NFIC antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG42974 anti-NFIC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse skeletal muscle tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42974 anti-NFIC antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42974 anti-NFIC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat pancreas muscle tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42974 anti-NFIC antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG42974 anti-NFIC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42974 anti-NFIC antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG42974 anti-NFIC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse skeletal muscle tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42974 anti-NFIC antibody at 2 µg/ml dilution, overnight at 4°C.
