ARG57060
anti-NNMT antibody [11G11]
anti-NNMT antibody [11G11] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [11G11] recognizes NNMT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 11G11 |
| Isotype | IgG2a, kappa |
| Target Name | NNMT |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-264 of Human NNMT. |
| Conjugation | Un-conjugated |
| Alternate Names | Nicotinamide N-methyltransferase; EC 2.1.1.1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NNMT |
| Gene Full Name | nicotinamide N-methyltransferase |
| Background | N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. [UniProt] |
| Calculated MW | 30 kDa |
Images (2) Click the Picture to Zoom In
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ARG57060 anti-NNMT antibody [11G11] FACS image
Flow Cytometry: HeLa cell line stained with ARG57060 anti-NNMT antibody [11G11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57060 anti-NNMT antibody [11G11] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) A549 cell lysate stained with ARG57060 anti-NNMT antibody [11G11] at 1:1000.
