ARG64477
anti-NOVA1 antibody
anti-NOVA1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes NOVA1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NOVA1 |
| Antigen Species | Human |
| Immunogen | C-REMPQNVAKTEPVS |
| Conjugation | Un-conjugated |
| Alternate Names | Paraneoplastic Ri antigen; Neuro-oncological ventral antigen 1; Nova-1; Onconeural ventral antigen 1; RNA-binding protein Nova-1; Ventral neuron-specific protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Neuroscience antibody |
| Calculated MW | 52 kDa |
Images (4) Click the Picture to Zoom In
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ARG64477 anti-NOVA1 antibody WB image
Western Blot: Human Breast Cancer lysate (35 µg protein in RIPA buffer) stained with ARG64477 anti-NOVA1 antibody at 0.01 µg/ml dilution.
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ARG64477 anti-NOVA1 antibody WB image
Western Blot: 1). Mock transfection; 2) NOVA1 (RC210407) expressing plasmid transfected HEK293 cell lysate standed with ARG64477 anti-NOVA1 antibody
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ARG64477 anti-NOVA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64477 anti-NOVA1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64477 anti-NOVA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64477 anti-NOVA1 antibody at 5 µg/ml dilution followed by AP-staining.
