ARG59878
anti-NPHS2 / Podocin antibody
anti-NPHS2 / Podocin antibody for IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NPHS2 / Podocin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Chk |
| Tested Application | IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NPHS2 / Podocin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 368-383 of Human NPHS2 / Podocin. (KPVEPLNPKKKDSPML) |
| Conjugation | Un-conjugated |
| Alternate Names | SRN1; Podocin; PDCN |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NPHS2 |
| Gene Full Name | nephrosis 2, idiopathic, steroid-resistant (podocin) |
| Background | This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. [UniProt] |
| Cellular Localization | Isoform 1: Cell membrane; Peripheral membrane protein. [UniProt] |
| Calculated MW | 42 kDa |
| PTM | Isoform 2 is glycosylated. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG59878 anti-NPHS2 / Podocin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59878 anti-NPHS2 / Podocin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59878 anti-NPHS2 / Podocin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59878 anti-NPHS2 / Podocin antibody at 1 µg/ml, overnight at 4°C.
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ARG59878 anti-NPHS2 / Podocin antibody WB image
Western blot: 50 ug of sample under reducing conditions. 293T whole cell lysate stained with ARG59878 anti-NPHS2 / Podocin antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59878 anti-NPHS2 / Podocin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59878 anti-NPHS2 / Podocin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59878 anti-NPHS2 / Podocin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59878 anti-NPHS2 / Podocin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59878 anti-NPHS2 / Podocin antibody WB image
Western blot: 50 ug of sample under reducing conditions. Rat kidney tissue lysate stained with ARG59878 anti-NPHS2 / Podocin antibody at 0.5 µg/ml, overnight at 4°C.
