ARG42028

anti-NSF antibody

anti-NSF antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NSF
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NSF
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-250 of Human NSF (NP_006169.2).
Conjugation Un-conjugated
Alternate Names SKD2; N-ethylmaleimide-sensitive fusion protein; Vesicular-fusion protein NSF; Vesicle-fusing ATPase; NEM-sensitive fusion protein; EC 3.6.4.6

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:2000
IPAssay-dependent
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat brain
Observed Size ~ 80 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18195 Mouse NSF

GeneID: 4905 Human NSF

GeneID: 60355 Rat NSF

Gene Symbol NSF
Gene Full Name N-ethylmaleimide-sensitive factor
Function Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity). [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 83 kDa
PTM Phosphorylation at Ser-569 interferes with homohexamerization. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG42028 anti-NSF antibody WB image

    Western blot: 25 µg of Rat brain lysate stained with ARG42028 anti-NSF antibody at 1:1000 dilution.