ARG44884
anti-NSMCE1 antibody
anti-NSMCE1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes NSMCE1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | NSMCE1 |
| Antigen Species | Human |
| Epitope | MQGSTRRMGV MTDVHRRFLQ LLMTHGVLEE WDVKRLQTHC YKVHDRNATV DKLEDFINNI NSVLESLYIE IKRGVTEDDG RPIYALVNLA TTSISKMATD FAENELDLFR KALELIIDSE TGFASSTNIL NLVDQLKGKK MRKKEAEQVL QKFVQNKWLI EKEGEFTLHG RAILEMEQYI RETYPDAVKI CNICHSLLIQ GQSCETCGIR MHLPCVAKYF QSNAEPRCPH CNDYWPHEIP KVFDPEKERE SGVLKSNKKS LRSRQH |
| Conjugation | Un-conjugated |
| Alternate Names | NSMCE1; NSE1 Homolog, SMC5-SMC6 Complex Component; NSE1; Non-Structural Maintenance Of Chromosomes Element 1 Homolog; Non-SMC Element 1 Homolog; Non-SMC Element 1 Homolog (S. Cerevisiae); EC 2.3.2.27 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Preservative | 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8WV22 Human Non-structural maintenance of chromosomes element 1 homolog |
|---|---|
| Gene Symbol | NSMCE1 |
| Gene Full Name | NSE1 Homolog, SMC5-SMC6 Complex Component |
| Background | Enables protein dimerization activity and ubiquitin protein ligase activity. Involved in positive regulation of response to DNA damage stimulus. Located in nucleoplasm. Part of Smc5-Smc6 complex. [provided by Alliance of Genome Resources, Apr 2022] |
| Function | MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. [Uniprot] |
| Cellular Localization | Chromosome, Nucleus, Telomere. [Uniprot] |
| PTM | Phosphoprotein, Ubl conjugation. [Uniprot] |
Images (3) Click the Picture to Zoom In
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ARG44884 anti-NSMCE1 antibody IHC-P image
Immunohistochemistry: Human heart muscle stained with ARG44884 anti-NSMCE1 antibody at 5 µg/mL dilution.
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ARG44884 anti-NSMCE1 antibody WB image
Western blot: Jurkat stained with ARG44884 anti-NSMCE1 antibody at 1 µg/mL dilution.
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ARG44884 anti-NSMCE1 antibody IP image
Immunoprecipitation: Jurkat lysate immunoprecipitated with 2.5 µg of ARG44884 anti-NSMCE1 antibody.
