ARG66955
anti-Nestin antibody
anti-Nestin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Nestin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Nestin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within the N-terminal region of Human Nestin. |
| Conjugation | Un-conjugated |
| Alternate Names | NES; Nestin FLJ21841; Nbla00170 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NES |
| Gene Full Name | nestin |
| Background | This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. |
| Function | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Cellular Localization | Intermediate filament |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody |
| Calculated MW | 177 kDa |
| PTM | Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation |
Images (5) Click the Picture to Zoom In
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ARG66955 anti-Nestin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded human cancer tissue stained with ARG66955 anti-Nestin antibody at 1:100 dilution.
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ARG66955 anti-Nestin antibody ICC/IF image
Immunofluorescence: Neuro2A cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained withARG66955 anti-Nestin antibody (red) at 1:200 dilution at 4°C for overnight. DAPI (blue) was used for nuclear staining.
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ARG66955 anti-Nestin antibody WB image
Western blot: SK-N-SH stained with ARG66955 anti-Nestin antibody at 1:1000 dilution.
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ARG66955 anti-Nestin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded human cancer tissue stained with ARG66955 anti-Nestin antibody at 1:100 dilution.
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ARG66955 anti-Nestin antibody WB image
Western blot: Nestin stained with ARG66955 anti-Nestin antibody at 1:1000 dilution.
