ARG64860

anti-Netrin 1 antibody

anti-Netrin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Netrin 1
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application ICC/IF, IHC-P
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Netrin 1
Antigen Species Human
Immunogen KFQQREKKGKCKKA
Conjugation Un-conjugated
Alternate Names Epididymis tissue protein Li 131P; Netrin-1; NTN1L

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
IHC-PAssay - dependent
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9423 Human NTN1

Swiss-port # O95631 Human Netrin-1

Background Netrin is included in a family of laminin-related secreted proteins. The function of this gene has not yet been defined; however, netrin is thought to be involved in axon guidance and cell migration during development. Mutations and loss of expression of netrin suggest that variation in netrin may be involved in cancer development. [provided by RefSeq, Jul 2008]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 68 kDa

Images (2) Click the Picture to Zoom In

  • ARG64860 anti-Netrin 1 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64860 anti-Netrin 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG64860 anti-Netrin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64860 anti-Netrin 1 antibody at 3.75 µg/ml dilution followed by AP-staining.