ARG22289
anti-Neuroligin 3 antibody [S110-29]
anti-Neuroligin 3 antibody [S110-29] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S110-29] recognizes Neuroligin 3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects ~110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S110-29 |
| Isotype | IgG1 |
| Target Name | Neuroligin 3 |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 730-848 (intracellular C-terminus) of Rat Neuroligin 3. Mouse: 99% identity (118/119 around aa. identical). Human: 98% identity (116/119 around aa. identical); ~60% identity with Neuroligin 1; ~40% identity with Neuroligin-2. |
| Conjugation | Un-conjugated |
| Alternate Names | Gliotactin homolog; Neuroligin-3; HNL3 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol |
| Preservative | 0.1% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Nlgn3 |
| Gene Full Name | neuroligin 3 |
| Background | This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Oct 2009] |
| Function | Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system (By similarity). [UniProt] |
| Cellular Localization | Cell Junction, Cell membrane, Synapse |
| Calculated MW | 94 kDa |
Images (2) Click the Picture to Zoom In
-
ARG22289 anti-Neuroligin 3 antibody [S110-29] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22289 anti-Neuroligin 3 antibody [S110-29] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22289 anti-Neuroligin 3 antibody [S110-29] (D) Composite.
-
ARG22289 anti-Neuroligin 3 antibody [S110-29] WB image
Western blot: 15 µg of Mouse Brain Membrane. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: ARG22289 anti-Neuroligin 3 antibody [S110-29] at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT.
