ARG66560
anti-Nodal antibody
anti-Nodal antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Nodal |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Nodal |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Human NODAL. |
| Conjugation | Un-conjugated |
| Alternate Names | Nodal homolog; HTX5 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 40 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NODAL |
| Gene Full Name | nodal growth differentiation factor |
| Background | The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008] |
| Function | Essential for mesoderm formation and axial patterning during embryonic development. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 40 kDa |
Images (2) Click the Picture to Zoom In
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ARG66560 anti-Nodal antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human brain tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66560 anti-Nodal antibody at room temperature and detected using an HRP conjugacompact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG66560 anti-Nodal antibody WB image
Western blot: HeLa whole cell lysate stained with ARG66560 anti-Nodal antibody.
