ARG65657
anti-OCT4 antibody
anti-OCT4 antibody for Western blot and Human,Mouse
Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes OCT4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Target Name | OCT4 |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide around the internal region of Mouse OCT4 (C-DRPNAVKLEKVEP). |
| Conjugation | Un-conjugated |
| Alternate Names | OTF-3; OCT4; OCT3; Octamer-binding protein 4; OTF3; Oct-3; Oct-4; OTF4; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Octamer-binding protein 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20263 Mouse POU domain, class 5, transcription factor 1 Swiss-port # Q01860 Human POU domain, class 5, transcription factor 1 |
|---|---|
| Gene Symbol | Pou5f1 |
| Gene Full Name | POU domain, class 5, transcription factor 1 |
| Background | The protein encoded by this gene belongs to the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein plays a key role in embryonic development and stem cell pluripotency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. [UniProt] |
| Research Area | Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 38.2 kDa (NP_038661.2) |
| PTM | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. |
Images (2) Click the Picture to Zoom In
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ARG65657 anti-OCT4 antibody WB image
Western blot: 35 μg of NIH3T3 lysate stained with ARG65657 anti-OCT4 antibody at 1 μg/ml dilution (1 hour incubation).
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ARG65657 anti-OCT4 antibody WB image
Western blot: 35 µg of HepG2 cell lysate (in RIPA buffer) stained with ARG65657 anti-OCT4 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
