ARG64115

anti-ORP5 antibody

anti-ORP5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ORP5
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms. Variants (NP_663613.1; NP_001137535.1) encode the same isoform.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ORP5
Antigen Species Human
Immunogen KEEAFLRRRFSLC
Conjugation Un-conjugated
Alternate Names Oxysterol-binding protein-related protein 5; OBPH1; ORP-5; OSBP-related protein 5; ORP5; Oxysterol-binding protein homolog 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.2 - 0.6 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 114879 Human OSBPL5

Swiss-port # Q9H0X9 Human Oxysterol-binding protein-related protein 5

Background This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that play a key role in the maintenance of cholesterol balance in the body. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. This gene has been shown to be imprinted, with preferential expression from the maternal allele only in placenta. Transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 99 kDa

Images (3) Click the Picture to Zoom In

  • ARG64115 anti-ORP5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64115 anti-ORP5 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64115 anti-ORP5 antibody WB image

    Western blot: 35 µg of Human spleen lysate (in RIPA buffer) stained with ARG64115 anti-ORP5 antibody at 0.2 µg/ml dilution and incubated at RT for 1 hour.

  • ARG64115 anti-ORP5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64115 anti-ORP5 antibody at 5 µg/ml dilution followed by AP-staining.