ARG56974

anti-OSTF1 antibody [9G4]

anti-OSTF1 antibody [9G4] for Flow cytometry,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [9G4] recognizes OSTF1
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 9G4
Isotype IgG2a, kappa
Target Name OSTF1
Antigen Species Human
Immunogen Recombinant fragment of Human OSTF1.
Conjugation Un-conjugated
Alternate Names SH3P2; bA235O14.1; Osteoclast-stimulating factor 1; OSF

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26578 Human OSTF1

Swiss-port # Q92882 Human Osteoclast-stimulating factor 1

Gene Symbol OSTF1
Gene Full Name osteoclast stimulating factor 1
Background Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM, Mar 2008]
Function Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity. [UniProt]
Calculated MW 24 kDa

Images (3) Click the Picture to Zoom In

  • ARG56974 anti-OSTF1 antibody [9G4] FACS image

    Flow Cytometry: HeLa cell line stained with ARG56974 anti-OSTF1 antibody [9G4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG56974 anti-OSTF1 antibody [9G4] WB image

    Western blot: 40 µg of 1) HepG2 cell lysate, 2) MCF7 cell lysate, 3) Jurkat cell lysate, 4) LnCap cell lysate stained with ARG56974 anti-OSTF1 antibody [9G4] at 1:1000.

  • ARG56974 anti-OSTF1 antibody [9G4] WB image

    Western blot: 10 µg of 1) 293T cell lysate, 2) OSTF1 Transfected 293T cell lysate stained with ARG56974 anti-OSTF1 antibody [9G4] at 1:1000.