ARG64159
anti-P4HA1 antibody
anti-P4HA1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes P4HA1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000908.2 and NP_001017962.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | P4HA1 |
| Antigen Species | Human |
| Immunogen | C-DFARKDEPDAFKE |
| Conjugation | Un-conjugated |
| Alternate Names | Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; P4HA; 4-PH alpha-1; EC 1.14.11.2; Prolyl 4-hydroxylase subunit alpha-1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | HEK293 | ||||||
| Observed Size | ~ 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P13674 Human Prolyl 4-hydroxylase subunit alpha-1 |
|---|---|
| Background | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 61 kDa |
Images (3) Click the Picture to Zoom In
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ARG64159 anti-P4HA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human skin tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64159 anti-P4HA1 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64159 anti-P4HA1 antibody WB image
Western blot: 35 µg of HEK293 cell lysate (in RIPA buffer) stained with ARG64159 anti-P4HA1 antibody at 0.2 µg/ml dilution and incubated at RT for 1 hour.
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ARG64159 anti-P4HA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64159 anti-P4HA1 antibody at 5 µg/ml dilution followed by AP-staining.
