ARG57886
anti-PBK / TOPK antibody
anti-PBK / TOPK antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PBK / TOPK |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PBK / TOPK |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-322 of Human PBK / TOPK (NP_060962.2). |
| Conjugation | Un-conjugated |
| Alternate Names | T-LAK cell-originated protein kinase; Nori-3; Spermatogenesis-related protein kinase; PDZ-binding kinase; EC 2.7.12.2; Lymphokine-activated killer T-cell-originated protein kinase; HEL164; SPK; TOPK; MAPKK-like protein kinase; Cancer/testis antigen 84; CT84 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | SW480 | ||||||
| Observed Size | 39 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96KB5 Human Lymphokine-activated killer T-cell-originated protein kinase |
|---|---|
| Gene Symbol | PBK |
| Gene Full Name | PDZ binding kinase |
| Background | This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | Phosphorylated; in a cell-cycle dependent manner at mitosis. [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG57886 anti-PBK / TOPK antibody WB image
Western blot: 25 µg of SW480 cell lysate stained with ARG57886 anti-PBK / TOPK antibody at 1:1000 dilution.
