Overview

Product Description	Rabbit Polyclonal antibody recognizes PDHB
Tested Reactivity	Hu, Ms, Rat
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PDHB
Antigen Species	Human
Immunogen	Recombinant protein of Human PDHB.
Conjugation	Un-conjugated
Alternate Names	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial; PDHBD; PDHE1-B; PHE1B; EC 1.2.4.1

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 289950 Rat PDHB GeneID: 5162 Human PDHB GeneID: 68263 Mouse PDHB Swiss-port # P11177 Human Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Swiss-port # P49432 Rat Pyruvate dehydrogenase E1 component subunit beta, mitochondrial Swiss-port # Q9D051 Mouse Pyruvate dehydrogenase E1 component subunit beta, mitochondrial More
Gene Symbol	PDHB
Gene Full Name	pyruvate dehydrogenase (lipoamide) beta
Background	The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012]
Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt]
Calculated MW	39 kDa

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