ARG65748
anti-PDI antibody
anti-PDI antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDI |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDI |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to a region of Human PDI (NP_006840.2). |
| Conjugation | Un-conjugated |
| Alternate Names | PDA2; Pancreas-specific protein disulfide isomerase; PDIR; PDIP; PDI; Protein disulfide-isomerase A2; PDIp; EC 5.3.4.1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa, MCF-7, PANC, HepG2, HUVEC, NIH/3T3, L929, F9, Jurkat, A431, A549, Mouse liver tissue, Mouse kidney tissue, Mouse brain tissue, Human lung carcinoma tissue, Human kidney, Human brain, Human liver, Human colon carcinoma tissue |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | TBS (pH 7.4), 0.05% Sodium azide, 40% Glycerol and 1% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol and 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDIA2 |
| Gene Full Name | protein disulfide isomerase family A, member 2 |
| Background | Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]). [supplied by OMIM, Mar 2008] |
| Function | Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | The disulfide-linked homodimer exhibits an enhanced chaperone activity. Glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG65748 anti-PDI antibody WB image
Western blot: 30 µg of MCF-7, Mouse liver, and MEF lysates stained with ARG65748 anti-PDI antibody at 1:500 dilution.
Customer's Feedback
Excellent
Review for anti-PDI antibody
Application：WB
Sample：Mouse liver
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-PDI antibody
Application：WB
Sample：MCF-7
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
