ARG40976
anti-PDXK antibody [1007CT2.1.1]
anti-PDXK antibody [1007CT2.1.1] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes PDXK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1007CT2.1.1 |
| Isotype | IgG1, kappa |
| Target Name | PDXK |
| Antigen Species | Human |
| Immunogen | Recombinant protein between aa. 1-312 of Human PDXK. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-1a; C21orf97; EC 2.7.1.35; C21orf124; PRED79; PNK; Pyridoxine kinase; PKH; Pyridoxal kinase |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | 293 | ||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDXK |
| Gene Full Name | pyridoxal (pyridoxine, vitamin B6) kinase |
| Background | The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Required for synthesis of pyridoxal-5-phosphate from vitamin B6. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 35 kDa |
Images (2) Click the Picture to Zoom In
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ARG40976 anti-PDXK antibody IHC-P image
Immunohistochemistry: Paraformaldehyde-fixed and paraffin-embedded Human testis tissue. Tissue was blocked with 3% BSA for 0.5 hour at room temperature. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG40976 anti-PDXK antibody at 1:25 dilution for 1 hours at 37°C.
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ARG40976 anti-PDXK antibody WB image
Western blot: 20 µg of 293 cell lysate stained with ARG40976 anti-PDXK antibody at 1:4000 dilution.
