ARG42606
anti-PFKM antibody
anti-PFKM antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody recognizes PFKM |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Target Name | PFKM |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human PFKM. |
| Conjugation | Un-conjugated |
| Alternate Names | PFK-A; 6-phosphofructokinase type A; PPP1R122; PFKX; ATP-dependent 6-phosphofructokinase, muscle type; EC 2.7.1.11; Phosphofructo-1-kinase isozyme A; PFK1; ATP-PFK; GSD7; PFK-1; Phosphohexokinase; PFK-M; PFKA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | PC-3M | ||||||||||
| Observed Size | ~ 82 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | TBS (pH 7.4), 0.05% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PFKM |
| Gene Full Name | phosphofructokinase, muscle |
| Background | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | GlcNAcylation decreases enzyme activity. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG42606 anti-PFKM antibody ICC/IF image
Immunofluorescence: SH-SY5Y cells were fixed in paraformaldehyde and permeabilized with 0.25% Triton X100/PBS. Cells were stained with ARG42606 anti-PFKM antibody (green). DAPI (blue) for nuclear staining.
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ARG42606 anti-PFKM antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse skeletal muscle tissue stained with ARG42606 anti-PFKM antibody and counter stained with hematoxylin.
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ARG42606 anti-PFKM antibody WB image
Western blot: PC-3M cell lysate stained with ARG42606 anti-PFKM antibody at 1:500 dilution.
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ARG42606 anti-PFKM antibody FACS image
Flow Cytometry: SH-SY5Y cells stained with ARG42606 anti-PFKM antibody (right histogram) at 1:100 dilution or without primary antibody as control (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
