ARG42634
anti-PFKM antibody
anti-PFKM antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PFKM |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PFKM |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human PFKM. |
| Conjugation | Un-conjugated |
| Alternate Names | PFK-A; 6-phosphofructokinase type A; PPP1R122; PFKX; ATP-dependent 6-phosphofructokinase, muscle type; EC 2.7.1.11; Phosphofructo-1-kinase isozyme A; PFK1; ATP-PFK; GSD7; PFK-1; Phosphohexokinase; PFK-M; PFKA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HeLa | ||||||||
| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PFKM |
| Gene Full Name | phosphofructokinase, muscle |
| Background | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | GlcNAcylation decreases enzyme activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG42634 anti-PFKM antibody WB image
Western blot: HeLa cell lysate stained with ARG42634 anti-PFKM antibody.
Specific References
Ochratoxin A-enhanced glycolysis induces inflammatory responses in human gastric epithelium cells through mTOR/HIF-1α signaling pathway
WB / Human
