ARG56306
anti-PFKM antibody
anti-PFKM antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PFKM |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PFKM |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human PFKM |
| Conjugation | Un-conjugated |
| Alternate Names | PFK-A; 6-phosphofructokinase type A; PPP1R122; PFKX; ATP-dependent 6-phosphofructokinase, muscle type; EC 2.7.1.11; Phosphofructo-1-kinase isozyme A; PFK1; ATP-PFK; GSD7; PFK-1; Phosphohexokinase; PFK-M; PFKA; Fructose 6 Phosphate Kinase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | 22Rv-1 | ||||||
| Observed Size | ~ 90 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PFKM |
| Gene Full Name | phosphofructokinase, muscle |
| Background | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009] |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProt] |
| Calculated MW | 85 kDa |
| PTM | GlcNAcylation decreases enzyme activity. |
Images (2) Click the Picture to Zoom In
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ARG56306 anti-PFKM antibody ICC/IF image
Immunofluorescence: L929 cells stained with ARG56306 anti-PFKM antibody at 1:100 dilution.
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ARG56306 anti-PFKM antibody WB image
Western blot: 22Rv-1 cell lysate stained with ARG56306 anti-PFKM antibody.
