ARG54874
anti-PFKP antibody
anti-PFKP antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PFKP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PFKP |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 754-784 (C-terminus) of Human PFKP. |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphohexokinase; PFK-C; EC 2.7.1.11; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; ATP-PFK; ATP-dependent 6-phosphofructokinase, platelet type; PFK-P; PFKF |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | T47D |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q01813 Human ATP-dependent 6-phosphofructokinase, platelet type |
|---|---|
| Gene Symbol | PFKP |
| Gene Full Name | phosphofructokinase, platelet |
| Background | The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM, Mar 2008] |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProt] |
| Cellular Localization | Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}. |
| Highlight | Related products: Anti-Rabbit IgG secondary antibodies; Related news: Vascular development is regulated by FGF-dependent metabolic control |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 86 kDa |
| PTM | GlcNAcylation decreases enzyme activity. |
Images (2) Click the Picture to Zoom In
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ARG54874 anti-PFKP antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast carcinoma tissue stained with ARG54874 anti-PFKP antibody.
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ARG54874 anti-PFKP antibody WB image
Western blot: 35 µg of T47D cell lysate stained with ARG54874 anti-PFKP antibody.
