Overview

Product Description	Rabbit Polyclonal antibody recognizes PHOX2B
Tested Reactivity	Hu, Ms
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PHOX2B
Antigen Species	Human
Immunogen	Synthetic peptide of Human PHOX2B.
Conjugation	Un-conjugated
Alternate Names	NBPhox; PHOX2B homeodomain protein; Paired-like homeobox 2B; Neuroblastoma Phox; Paired mesoderm homeobox protein 2B; PMX2B; NBLST2

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:100 - 1:500 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Neuro-2a
Observed Size	~ 35 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 18935 Mouse PHOX2B GeneID: 8929 Human PHOX2B Swiss-port # O35690 Mouse Paired mesoderm homeobox protein 2B Swiss-port # Q99453 Human Paired mesoderm homeobox protein 2B
Gene Symbol	PHOX2B
Gene Full Name	paired-like homeobox 2b
Background	The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq, Jul 2008]
Function	Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element. [UniProt]
Cellular Localization	Nucleus. [UniProt]
Calculated MW	32 kDa

Images (1) Click the Picture to Zoom In