ARG45944
anti-PIK3C2A antibody
anti-PIK3C2A antibody for ICC/IF,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PIK3C2A |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PIK3C2A |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human PIK3C2A. |
| Conjugation | Un-conjugated |
| Alternate Names | PIK3C2A; Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Alpha; EC 2.7.1.154; PI3K-C2-alpha; CPK; PI3-K-C2(ALPHA); Phosphoinositide 3-kinase-C2-alpha; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; PtdIns-3-kinase C2 subunit alpha; PI3-K-C2A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 191 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O00443 Human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alp Swiss-port # Q61194 Mouse Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alp |
|---|---|
| Gene Symbol | PIK3C2A |
| Gene Full Name | Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Alpha |
| Background | The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008] |
| Function | Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components. [UniProt] |
| Cellular Localization | Nucleus; Cell membrane; Cytoplasmic vesicle; Cytoplasm; Golgi apparatus / Trans-Golgi network; Membrane. [UniProt] |
| Calculated MW | 191 kDa |
| PTM | Phosphoprotein; Acetylation. [UniProt]. [UniProt] |
