ARG51819
anti-PLC gamma 2 phospho (Tyr1217) antibody
anti-PLC gamma 2 phospho (Tyr1217) antibody for IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PLC gamma 2 phospho (Tyr1217) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLC gamma 2 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1217 (F-L-Y(p)-D-T) derived from Human PLCγ2. |
| Conjugation | Un-conjugated |
| Alternate Names | Phospholipase C-gamma-2; PLC-IV; Phosphoinositide phospholipase C-gamma-2; PLC-gamma-2; Phospholipase C-IV; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; FCAS3; APLAID; EC 3.1.4.11 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PLCG2 |
| Gene Full Name | phospholipase C, gamma 2 (phosphatidylinositol-specific) |
| Background | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. |
| Function | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 148 kDa |
| PTM | Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity. |
Images (1) Click the Picture to Zoom In
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ARG51819 anti-PLC gamma 2 phospho (Tyr1217) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51819 anti-PLC gamma 2 phospho (Tyr1217) antibody (left) or the same antibody preincubated with blocking peptide (right).
