ARG55680
anti-PLOD1 antibody
anti-PLOD1 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PLOD1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLOD1 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 66-94 (N-terminus) of Human PLOD1. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 1.14.11.4; EDS6; Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1; Lysyl hydroxylase 1; LH; PLOD; LLH; LH1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | A431 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q02809 Human Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 |
|---|---|
| Gene Symbol | PLOD1 |
| Gene Full Name | procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 |
| Background | Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq, Jul 2008] |
| Function | Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. [UniProt] |
| Cellular Localization | Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side |
| Calculated MW | 84 kDa |
Images (1) Click the Picture to Zoom In
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ARG55680 anti-PLOD1 antibody WB image
Western blot: 20 µg of A431 cell lysate stained with ARG55680 anti-PLOD1 antibody at 1:1000 dilution.
