ARG57043
anti-PMVK antibody [21C10]
anti-PMVK antibody [21C10] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [21C10] recognizes PMVK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 21C10 |
| Isotype | IgG2a, kappa |
| Target Name | PMVK |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-192 of Human PMVK. |
| Conjugation | Un-conjugated |
| Alternate Names | PMKase; PMKASE; hPMK; PMKA; HUMPMKI; EC 2.7.4.2; Phosphomevalonate kinase; PMK |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PMVK |
| Gene Full Name | phosphomevalonate kinase |
| Background | This gene encodes a peroxisomal enzyme that catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate, the fifth reaction of the cholesterol biosynthetic pathway. Studies in rat show that the message level and the enzyme activity of this protein is regulated by sterol, and that this regulation is coordinated with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. [provided by RefSeq, Sep 2011] |
| Calculated MW | 22 kDa |
Images (2) Click the Picture to Zoom In
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ARG57043 anti-PMVK antibody [21C10] FACS image
Flow Cytometry: LNCap cell line stained with ARG57043 anti-PMVK antibody [21C10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57043 anti-PMVK antibody [21C10] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) HepG2 cell lysate, 3) Jukat cell lysate, 4) MCF7 cell lysate, 5) A549 cell lysate stained with ARG57043 anti-PMVK antibody [21C10] at 1:1000.
