ARG45438
anti-POLD4 antibody
anti-POLD4 antibody for Western blot,ICC/IF,Flow cytometry and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes POLD4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POLD4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human POLD4. |
| Conjugation | Un-conjugated |
| Alternate Names | POLD4; DNA Polymerase Delta 4, Accessory Subunit; POLDS; P12; Polymerase (DNA-Directed), Delta 4, Accessory Subunit; Polymerase (DNA) Delta 4, Accessory Subunit; DNA Polymerase Delta Smallest Subunit P12; DNA Polymerase Delta Subunit 4; DNA Polymerase Delta Subunit P12 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 16 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POLD4 |
| Gene Full Name | DNA Polymerase Delta 4, Accessory Subunit |
| Background | This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] |
| Function | As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 12 kDa |
| PTM | Ubiquitinated; undergoes 'Lys-48'-linked ubiquitination in response to UV irradiation, leading to proteasomal degradation. [UniProt] |
