ARG45951
anti-POLG2 antibody
anti-POLG2 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes POLG2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POLG2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human POLG2. |
| Conjugation | Un-conjugated |
| Alternate Names | DNA Polymerase Gamma 2, Accessory Subunit; POLG2; MTPOLB; DNA polymerase subunit gamma-2, mitochondrial; DNA polymerase gamma accessory 55 kDa subunit; p55; Mitochondrial DNA polymerase accessory subunit; MtPolB; PolG-beta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UHN1 Human DNA polymerase subunit gamma-2, mitochondrial |
|---|---|
| Gene Symbol | POLG2 |
| Gene Full Name | DNA Polymerase Gamma 2, Accessory Subunit |
| Background | This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions. [provided by RefSeq, Jul 2008] |
| Function | Accessory subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Acts as an allosteric regulator of the holoenzyme activities. Enhances the polymerase activity and the processivity of POLG by increasing its interactions with the DNA template. Suppresses POLG exonucleolytic proofreading especially toward homopolymeric templates bearing mismatched termini. Binds to single-stranded DNA. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 55 kDa |
| PTM | Phosphoprotein. [UniProt]. [UniProt] |
