ARG57492
anti-PPIE antibody [17E8]
anti-PPIE antibody [17E8] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [17E8] recognizes PPIE |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 17E8 |
| Isotype | IgG1, kappa |
| Target Name | PPIE |
| Antigen Species | Human |
| Immunogen | Recombinant Human PPIE (aa. 1-301aa) purified from E. coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Cyclophilin-33; CYP-33; Cyclophilin E; PPIase E; EC 5.2.1.8; Peptidyl-prolyl cis-trans isomerase E; CYP33; Rotamase E |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UNP9 Human Peptidyl-prolyl cis-trans isomerase E |
|---|---|
| Gene Symbol | PPIE |
| Gene Full Name | peptidylprolyl isomerase E (cyclophilin E) |
| Background | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010] |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. [UniProt] |
| Calculated MW | 33 kDa |
Images (1) Click the Picture to Zoom In
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ARG57492 anti-PPIE antibody [17E8] WB image
Western blot: 40 µg of HeLa, Jurkat, 293T, A549, K562, MCF7 and LnCap cell lysates stained with ARG57492 anti-PPIE antibody [17E8] at 1:1000 dilution.
