ARG62602
anti-PR3 antibody [MCPR3-2]
anti-PR3 antibody [MCPR3-2] for Flow cytometry,ICC/IF,Western blot and Human
Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [MCPR3-2] recognizes PR3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MCPR3-2 |
| Isotype | IgG1 |
| Target Name | PR3 |
| Immunogen | HMC-1/PR3 cell granule extract |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.4.21.76; MBN; Neutrophil proteinase 4; PR-3; Wegener autoantigen; AGP7; CANCA; C-ANCA antigen; C-ANCA; Leukocyte proteinase 3; ACPA; PR3; NP-4; MBT; NP4; Myeloblastin; P29 |
Application Instructions
| Application Note | FACS: 0.1µg for 106 cells * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 1X PBS buffer with 0.1% sodium azide. |
| Preservative | 0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRTN3 |
| Gene Full Name | proteinase 3 |
| Function | Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. [UniProt] |
| Research Area | Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 28 kDa |
Clone References
A hydrophobic patch on proteinase 3, the target of autoantibodies in Wegener granulomatosis, mediates membrane binding via NB1 receptors.
FACS / Human
Capture-ELISA based on recombinant PR3 is sensitive for PR3-ANCA testing and allows detection of PR3 and PR3-ANCA/PR3 immunecomplexes.
WB / Human
