ARG45805

anti-PRAGMIN / SGK223 antibody

anti-PRAGMIN / SGK223 antibody for Flow cytometry,ICC/IF,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PRAGMIN / SGK223
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PRAGMIN / SGK223
Antigen Species Human
Immunogen Recombinant protein containing to human PRAGMIN / SGK223.
Conjugation Un-conjugated
Alternate Names PRAG1; PEAK1 Related, Kinase-Activating Pseudokinase 1; SgK223; Sugen Kinase 223; PRAGMIN; PEAK2; NACK; Inactive Tyrosine-Protein Kinase PRAG1; Pragmin, RND2 Effector Protein; PEAK Family Member 2; DKFZp761P0423; PEAK1-Related Kinase-Activating Pseudokinase 1; Tyrosine-Protein Kinase SgK223; Homolog Of Rat Pragma Of Rnd2; Tyrosine-Protein Kinase PRAG1; Pragmin; SGK223

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 200 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 157285 Human SGK223

Swiss-port # Q86YV5 Human Tyrosine-protein kinase SgK223

Gene Symbol PRAG1
Gene Full Name PEAK1 Related, Kinase-Activating Pseudokinase 1
Background This gene encodes an enzyme that belongs to the tyrosine protein kinase family. A similar protein in rat binds to Rho family GTPase 2 (Rnd2) and regulates neurite outgrowth via activation of Ras homolog gene family, member A (RhoA). [provided by RefSeq, Mar 2014]
Function Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By similarity). Promotes Src family kinase (SFK) signaling by regulating the subcellular localization of CSK, a negative regulator of these kinases, leading to the regulation of cell morphology and motility by a CSK-dependent mechanism (By similarity). Acts as a critical coactivator of Notch signaling (By similarity). [UniProt]
Cellular Localization Cell junction; Cytoplasm; Nucleus. [UniProt]
Calculated MW 150 kDa
PTM Phosphoprotein. [UniProt]