ARG53960
anti-PRR7 antibody [TRAP3/10]
anti-PRR7 antibody [TRAP3/10] for ICC/IF,Western blot and Human,Mouse,Rat
Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [TRAP3/10] recognizes PRR7 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Specificity | The clone TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | TRAP3/10 |
| Isotype | IgG2a |
| Target Name | PRR7 |
| Antigen Species | Human |
| Immunogen | Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253) |
| Conjugation | Un-conjugated |
| Alternate Names | Synaptic proline-rich membrane protein; Proline-rich protein 7 |
Application Instructions
| Application Suggestion |
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| Application Note | ICC/IF: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: Murine brain lysate (red. Laemmli buffer) |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRR7 |
| Gene Full Name | proline rich 7 (synaptic) |
| Background | PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse. |
| Research Area | Neuroscience antibody |
| Calculated MW | 31 kDa |
Images (2) Click the Picture to Zoom In
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ARG53960 anti-PRR7 antibody [TRAP3/10] ICC/IF image
Immunofluorescence: HEK-293 cells cotransfected with PRR7 / TRAP3 and GFP-PSD-95 stained with ARG53960 anti-PRR7 antibody [TRAP3/10] (red)
Co-stained with GFP (green). Cell nuclei was stained with DAPI (blue). -
ARG53960 anti-PRR7 antibody [TRAP3/10] WB image
Western blot: Murine brain lysate stained with ARG53960 anti-PRR7 antibody [TRAP3/10].
