Overview

Product Description	Rabbit Polyclonal antibody recognizes PSMD14
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PSMD14
Antigen Species	Human
Immunogen	Synthetic peptide of Human PSMD14.
Conjugation	Un-conjugated
Alternate Names	RPN11; 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome-associated PAD1 homolog 1; POH1; PAD1; EC 3.4.19.-; 26S proteasome regulatory subunit RPN11

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:1000 - 1:5000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size	~ 35 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative	0.01% Sodium azide
Stabilizer	40% Glycerol and 0.05% BSA
Concentration	Batch dependent
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 10213 Human PSMD14 GeneID: 59029 Mouse PSMD14 Swiss-port # O00487 Human 26S proteasome non-ATPase regulatory subunit 14 Swiss-port # O35593 Mouse 26S proteasome non-ATPase regulatory subunit 14
Gene Symbol	PSMD14
Gene Full Name	proteasome 26S subunit, non-ATPase 14
Background	This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]
Function	Metalloprotease component of the 26S proteasome that specifically cleaves 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading. [UniProt]
Calculated MW	35 kDa

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