ARG41039
anti-PSMD8 antibody
anti-PSMD8 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PSMD8 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PSMD8 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 101-350 of Human PSMD8 (NP_002803.2). |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-91n; p31; HIP6; S14; 26S proteasome regulatory subunit S14; 26S proteasome non-ATPase regulatory subunit 8; 26S proteasome regulatory subunit RPN12; Rpn12; Nin1p; HYPF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse skeletal muscle | ||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P48556 Human 26S proteasome non-ATPase regulatory subunit 8 Swiss-port # Q9CX56 Mouse 26S proteasome non-ATPase regulatory subunit 8 |
|---|---|
| Gene Symbol | PSMD8 |
| Gene Full Name | proteasome 26S subunit, non-ATPase 8 |
| Background | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008] |
| Function | Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase. [UniProt] |
| Calculated MW | 40 kDa |
Images (2) Click the Picture to Zoom In
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ARG41039 anti-PSMD8 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41039 anti-PSMD8 antibody.
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ARG41039 anti-PSMD8 antibody WB image
Western blot: 25 µg of Mouse skeletal muscle lysate stained with ARG41039 anti-PSMD8 antibody at 1:1000 dilution.
