ARG63763
anti-PUM2 antibody
anti-PUM2 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Developmental Biology antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PUM2 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PUM2 |
| Antigen Species | Human |
| Immunogen | C-DQNRDLKQGDDDDSK |
| Conjugation | Un-conjugated |
| Alternate Names | PUML2; Pumilio-2; PUMH2; Pumilio homolog 2 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
| Research Area | Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 114 kDa |
Images (2) Click the Picture to Zoom In
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ARG63763 anti-PUM2 antibody WB image
Western blot: 10 µg of HEK293 cell lysate (in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag stained with ARG63763 anti-PUM2 antibody at 0.5 µg/ml dilution (Lane A); Mock-transfected HEK293 stained with primary antibodies (Lane B); Overexpressing HEK293 cell lysate stained with anti-DYKDDDDK Tag at 1:3000 dilution (Lane C). Primary antibodies were incubated at RT for 1 hour.
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ARG63763 anti-PUM2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63763 anti-PUM2 antibody at 10 µg/ml dilution followed by AP-staining.
