ARG45125
anti-Patched 1 antibody
anti-Patched 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes Patched 1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Patched 1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human Patched 1. |
| Conjugation | Un-conjugated |
| Alternate Names | PTCH1; Patched 1; BCNS; PTCH; Protein Patched Homolog 1; NBCCS; PTC1; PTC; Patched Homolog 1 (Drosophila); Patched (Drosophila) Homolog; Patched Homolog (Drosophila); BCNS1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PTCH1 |
| Gene Full Name | Patched 1 |
| Background | This gene encodes a member of the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The encoded protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog. Following binding by one of the hedgehog ligands, the encoded protein is trafficked away from the primary cilium, relieving inhibition of the G-protein-coupled receptor smoothened, which results in activation of downstream signaling. Mutations of this gene have been associated with basal cell nevus syndrome and holoprosencephaly. [provided by RefSeq, Aug 2017] |
| Function | Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. [Uniprot] |
| Cellular Localization | Cell membrane; Membrane. [Uniprot] |
| Calculated MW | 161 kDa |
| PTM | Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation. [Uniprot] |
Images (3) Click the Picture to Zoom In
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ARG45125 anti-Patched 1 antibody IHC-P image
Immunohistochemistry: Human Cortex stained with ARG45125 anti-Patched 1 antibody at 8 μg/ml dilution.
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ARG45125 anti-Patched 1 antibody ICC/IF image
Immunofluorescence: HeLa stained with ARG45125 anti-Patched 1 antibody at 5 μg/ml dilution.
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ARG45125 anti-Patched 1 antibody WB image
Western blot: Mouse Fetal Kidney stained with ARG45125 anti-Patched 1 antibody at 2 μg/ml dilution.
