ARG63086
anti-Phosphotyrosine antibody [P-TYR-01]
anti-Phosphotyrosine antibody [P-TYR-01] for Flow cytometry,ICC/IF,Western blot and All
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [P-TYR-01] recognizes Phosphotyrosine |
|---|---|
| Tested Reactivity | All |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | The clone P-Tyr-01 detects tyrosine phosphorylation in activated cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | P-TYR-01 |
| Isotype | IgG1 |
| Target Name | Phosphotyrosine |
| Immunogen | Phosphotyrosine conjugated to bovine serum albumin. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
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Images (1) Click the Picture to Zoom In
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ARG63086 anti-Phosphotyrosine antibody [P-TYR-01] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG63086 anti-Phosphotyrosine antibody [P-TYR-01] (green).
Actin was stained with phalloidin-Alexa Fluor-567 (red), DNA was stained with DAPI (blue).
Clone References
Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization.
Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.
T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains.
Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.
