ARG10152
anti-Procalcitonin antibody [42]
anti-Procalcitonin antibody [42] for ELISA,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [42] recognizes Procalcitonin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 42 |
| Isotype | IgG2a |
| Target Name | Procalcitonin |
| Antigen Species | Human |
| Immunogen | Fragments of human PCT conjugated with carrier protein. |
| Conjugation | Un-conjugated |
| Alternate Names | CALC1; Calcitonin carboxyl-terminal peptide; CCP; PDN-21; CT |
Application Instructions
| Application Note | Sandwich ELISA: Recommended antibody pairs in sandwich immunoassay for procalcitonin (capture - detection): ARG10509 - ARG10152 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A affinity purified. |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 1.0-2.0 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CALCA |
| Gene Full Name | Calcitonin |
| Background | This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2014] |
| Function | Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Katacalcin is a potent plasma calcium-lowering peptide. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 14 kDa |
Images (4) Click the Picture to Zoom In
-
ARG10152 anti-Procalcitonin antibody [42] WB image
Western Blot: 100ng/lane Human recominbinant procalcitonin stained with 1) ARG10165 anti-Procalcitonin antibody [6F10]; 2)ARG10436 anti-Procalcitonin antibody [27A3]; 3)ARG10136 anti-Procalcitonin antibody [38F11]; 4) ARG10118 anti-Procalcitonin antibody [44D9]; 5) ARG10152 anti-Procalcitonin antibody [42].
-
ARG10152 anti-Procalcitonin antibody [42] Immuongen location image
The epitope of ARG10152 anti-Procalcitonin antibody [42] is located at a.a. 21-40 of Proalcitonin.
-
ARG10152 anti-Procalcitonin antibody [42] ELISA image
ELISA: Titration curve of ARG10152 in direct ELISA with N-terminal PCT conjugated with BSA - 0.02 µg/well.
-
ARG10152 anti-Procalcitonin antibody [42] sELISA image
Sandwich ELISA: Calibration curves for MAb combinations clone 16B5 - 42. The immunoassay was pre-coated with 1 μg/well of ARG10152 anti-Procalcitonin antibody [42] as the capture antibody. Recombinant human proalcitonin was added to the wells and incubated for 30 min at RT. After wash away unbonded reagents. A Eu3+-labeled detection antibody, ARG10509 anti-Calcitonin antibody [16B5] (0.1 μg/wel) was added and incubated.
