ARG40831
anti-Prohibitin antibody
anti-Prohibitin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Prohibitin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Hm |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Prohibitin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence at the C-terminus of Human Prohibitin (240-256aa KLEAAEDIAYQLSRSRN) |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-215; PHB1; Prohibitin; HEL-S-54e |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PHB |
| Gene Full Name | prohibitin |
| Background | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane. [UniProt] |
| Calculated MW | 30 kDa |
Images (6) Click the Picture to Zoom In
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ARG40831 anti-Prohibitin antibody ICC image
Immunocytochemistry: HeLa cells stained with ARG40831 anti-Prohibitin antibody.
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ARG40831 anti-Prohibitin antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG40831 anti-Prohibitin antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG40831 anti-Prohibitin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG40831 anti-Prohibitin antibody.
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ARG40831 anti-Prohibitin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40831 anti-Prohibitin antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG40831 anti-Prohibitin antibody WB image
Western blot: Rat lung, Rat skeletal muscle, Rat brain, Rat kidney, HeLa, MCF7, PC-12, A549 and SMMC-7721 cell lysates stained with ARG40831 anti-Prohibitin antibody.
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ARG40831 anti-Prohibitin antibody WB image
Western blot: 50 µg of sample under reducing conditions. NIH/3T3 whole cell lysate stained with ARG40831 anti-Prohibitin antibody at 0.5 µg/ml dilution, overnight at 4°C.
