ARG55648
anti-Prohibitin antibody
anti-Prohibitin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Prohibitin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Prohibitin |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Prohibitin. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-215; PHB1; Prohibitin; HEL-S-54e |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | PC-12 and PC-3 | ||||||||
| Observed Size | ~ 30 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PHB |
| Gene Full Name | prohibitin |
| Background | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. [UniProt] |
| Calculated MW | 30 kDa |
Images (2) Click the Picture to Zoom In
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ARG55648 anti-Prohibitin antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55648 anti-Prohibitin antibody.
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ARG55648 anti-Prohibitin antibody WB image
Western blot: PC-12 and PC-3 cell lysates stained with ARG55648 anti-Prohibitin antibody.
