ARG62603

anti-Prohibitin antibody [II-14-10]

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100 μl

349.00

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Overview

Product Description	Mouse Monoclonal antibody [II-14-10] recognizes Prohibitin
Tested Reactivity	Hu, Ms, Rat, Pig
Tested Application	FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host	Mouse
Clonality	Monoclonal
Clone	II-14-10
Isotype	IgG1
Target Name	Prohibitin
Antigen Species	Rat
Immunogen	BALB/C mice were injected with purified Recombinant Rat Prohibitin protein.
Conjugation	Un-conjugated
Alternate Names	HEL-215; PHB1; Prohibitin; HEL-S-54e

Application Instructions

Application Note	WB: 1/100 FACS: 1µg for 106 cells IHC-P: 1/50 - 1/100 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Buffer	PBS, 1% BSA and 0.05% Sodium azide
Preservative	0.05% Sodium azide
Stabilizer	1% BSA
Concentration	0.2 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 18673 Mouse PHB GeneID: 25344 Rat PHB GeneID: 5245 Human PHB Swiss-port # P35232 Human Prohibitin Swiss-port # P67778 Mouse Prohibitin Swiss-port # P67779 Rat Prohibitin More
Gene Symbol	Phb
Gene Full Name	prohibitin
Background	This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function	Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. [UniProt]
Highlight	Related products: Prohibitin antibodies; Anti-Mouse IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf
Research Area	Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW	30 kDa

Clone References

Identification of prohibitin 1 as a potential prognostic biomarker in human pancreatic carcinoma using modified aqueous two-phase partition system combined with 2D-MALDI-TOF-TOF-MS/MS.

Zhong N et al.
Tumour Biol., (2015)

Modulation of age-related insulin sensitivity by VEGF-dependent vascular plasticity in adipose tissues.

Honek J et al.
Proc Natl Acad Sci U S A., (2014)

Prohibitins function as endogenous ligands for Siglec-9 and negatively regulate TCR signaling upon ligation.

WB / Human

Yurugi H et al.
Biochem Biophys Res Commun., (2013)

GRSF1 regulates RNA processing in mitochondrial RNA granules.

WB / Human

Jourdain AA et al.
Cell Metab., (2013)

All-trans retinoic acid treatment is associated with prohibitin expression in renal interstitial fibrosis rats.

IHC / Rat

Zhou TB et al.
Int J Mol Sci., (2012)

Isoform-specific targeting of PKA to multivesicular bodies.

Day ME et al.
J Cell Biol., (2011)

Proteomic analysis of myocardial tissue from the border zone during early stage post-infarct remodelling in rats.

WB / Rat

Xiang F et al.
Eur J Heart Fail., (2011)

Mitochondrial comparative proteomics of human ovarian cancer cells and their platinum-resistant sublines.

WB, IHC-P / Human

Dai Z et al.
Proteomics., (2010)

PPARγ-induced cardiolipotoxicity in mice is ameliorated by PPARα deficiency despite increases in fatty acid oxidation.

IHC-Fr / Mouse

Son NH et al.
J Clin Invest., (2010)

Prohibitin as an oxidative stress biomarker in the eye.

WB / Bovine

Lee H et al.
Int J Biol Macromol., (2010)

Intrauterine growth restriction and postnatal high-protein diet affect the kidneys in adult rats.

WB / Rat

Shen Q et al.
Nutrition., (2011)

Extracellular growth factors and mitogens cooperate to drive mitochondrial biogenesis.

ICC/IF / Rat

Echave P et al.
J Cell Sci., (2009)

Leishmania cell surface prohibitin: role in host-parasite interaction.

WB / Mouse

Jain R et al.
Cell Microbiol., (2010)

Cellular vimentin content regulates the protein level of hepatitis C virus core protein and the hepatitis C virus production in cultured cells.

WB / Virus

Nitahara-Kasahara Y et al.
Virology., (2009)

A comparison of the synaptic proteome in human chronic schizophrenia and rat ketamine psychosis suggest that prohibitin is involved in the synaptic pathology of schizophrenia.

WB, ICC/IF, IHC-P / Human, Rat

Smalla KH et al.
Mol Psychiatry., (2008)

Development of the terminally differentiated state sensitizes epiphyseal chondrocytes to apoptosis through caspase-3 activation.

WB / Bird

Pucci B et al.
J Cell Physiol., (2007)

Differential regulation of human YY1 and caspase 7 promoters by prohibitin through E2F1 and p53 binding sites.

ChIP / Human

Joshi B et al.
Biochem J., (2007)