ARG41415
anti-Protein C antibody
anti-Protein C antibody for Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Protein C |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Protein C |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to D200-P461 of Human Protein C. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.4.21.69; Blood coagulation factor XIV; PC; THPH3; Vitamin K-dependent protein C; THPH4; APC; Autoprothrombin IIA; PROC1; Anticoagulant protein C |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | ~ 53 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PROC |
| Gene Full Name | protein C (inactivator of coagulation factors Va and VIIIa) |
| Background | This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombin-thrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.[provided by RefSeq, Dec 2009] |
| Function | Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. [UniProt] |
| Cellular Localization | Secreted. Golgi apparatus. Endoplasmic reticulum. [UniProt] |
| Calculated MW | 52 kDa |
| PTM | The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41415 anti-Protein C antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat liver and Mouse liver lysates stained with ARG41415 anti-Protein C antibody at 0.5 µg/ml dilution, overnight at 4°C.
