ARG54014
anti-Pyruvate Dehydrogenase E1 alpha subunit antibody
anti-Pyruvate Dehydrogenase E1 alpha subunit antibody for ICC/IF,Western blot and Human,Mouse
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody recognizes Pyruvate Dehydrogenase E1 alpha subunit |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Pyruvate Dehydrogenase E1 alpha subunit |
| Antigen Species | Human |
| Immunogen | Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A; EC 1.2.4.1; PDHE1-A type I; PDHCE1A; PDHAD; PDHA |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
| Preservative | 0.2% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08559 Human Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochond Swiss-port # P35486 Mouse Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochond |
|---|---|
| Gene Symbol | PDHA1 |
| Gene Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
| Background | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). |
| Function | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt] |
| Cellular Localization | Mitochondrion matrix |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 43 kDa |
| PTM | Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation. Acetylation alters the phosphorylation pattern. Deacetylated by SIRT3 (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody (green) at 1:100 dilution.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody WB image
Western blot: 30 µg of 293T and HeLa cell lysates stained with ARG54014 anti-Pyruvate Dehydrogenase E1 alpha subunit antibody at 1:1000 dilution.
Customer's Feedback
Good
Review for anti-Pyruvate Dehydrogenase E1 alpha subunit antibody
Application：WB
Sample：HepG2
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Pyruvate Dehydrogenase E1 alpha subunit antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:100
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
