ARG42524
anti-RAB32 antibody
anti-RAB32 antibody for ICC/IF,Western blot and Human,Mouse,Rat,Dog,Monkey
Overview
| Product Description | Goat Polyclonal antibody recognizes RAB32 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
| Tested Application | ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAB32 |
| Antigen Species | Human |
| Immunogen | Purified recombinant peptide within aa. 120 to the C-terminus of Human RAB32. |
| Conjugation | Un-conjugated |
| Alternate Names | Ras-related protein Rab-32 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | NIH/3T3 | ||||||
| Observed Size | ~ 32 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 20% Glycerol |
| Concentration | 4 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RAB32 |
| Gene Full Name | RAB32, member RAS oncogene family |
| Background | The protein encoded by this gene anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. The encoded protein also appears to be involved in autophagy and melanosome secretion. Variations in this gene may be linked to leprosy. [provided by RefSeq, Dec 2015] |
| Function | Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed:12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed:21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed:23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). [UniProt] |
| Cellular Localization | Mitochondrion. Mitochondrion outer membrane; Lipid-anchor. Cytoplasmic vesicle, phagosome. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Melanosome. Melanosome membrane. Note=Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211). The BLOC-3 complex, a heterodimer of HPS1 and HPS4 promotes its membrane localization (PubMed:23084991). [UniProt] |
| Calculated MW | 25 kDa |
Images (2) Click the Picture to Zoom In
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ARG42524 anti-RAB32 antibody ICC/IF image
Immunofluorescence: Primary RPE cells stained with ARG42524 anti-RAB32 antibody (green). Nuclear staining (blue).
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ARG42524 anti-RAB32 antibody WB image
Western blot: 100 µg of NIH/3T3 cell lysate stained with ARG42524 anti-RAB32 antibody at 1:500 dilution.
