ARG42511

anti-RAB5B antibody

anti-RAB5B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Dog,Human,Monkey,Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes RAB5B
Tested Reactivity Hu, Ms, Rat, Dog, Mk
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RAB5B
Antigen Species Mouse
Immunogen Purified recombinant peptide within aa. 115 to the C-terminus of Mouse RAB5B.
Conjugation Un-conjugated
Alternate Names Ras-related protein Rab-5B

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:250
IHC-Fr1:100 - 1:500
IHC-P1:100 - 1:500
WB1:250 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MDCK
Observed Size ~ 26 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 4 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19344 Mouse RAB5B

GeneID: 5869 Human RAB5B

Swiss-port # P61020 Human Ras-related protein Rab-5B

Swiss-port # P61021 Mouse Ras-related protein Rab-5B

Gene Symbol RAB5B
Gene Full Name RAB5B, member RAS oncogene family
Function Protein transport. Probably involved in vesicular traffic (By similarity). [UniProt]
Cellular Localization Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Note=Enriched in stage I melanosomes. [UniProt]
Calculated MW 24 kDa

Images (2) Click the Picture to Zoom In

  • ARG42511 anti-RAB5B antibody ICC/IF image

    Immunofluorescence: B6-RPE07 cells were stained with ARG42511 anti-RAB5B antibody (green) at 1:100 dilution. Nuclear staining (blue).

  • ARG42511 anti-RAB5B antibody WB image

    Western blot: MDCK cell lysate stained with ARG42511 anti-RAB5B antibody.