Overview

Product Description	Rabbit Polyclonal antibody recognizes RANGAP1
Tested Reactivity	Hu, Ms
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	RANGAP1
Antigen Species	Human
Immunogen	Recombinant protein of Human RANGAP1
Conjugation	Un-conjugated
Alternate Names	RANGAP; Fug1; RanGAP1; Ran GTPase-activating protein 1; SD

Application Instructions

Predict Reactivity Note	Rat
Application Suggestion	Tested Application Dilution WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Jurkat
Observed Size	~ 63 kDa

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 19387 Mouse RANGAP1 GeneID: 5905 Human RANGAP1 Swiss-port # P46060 Human Ran GTPase-activating protein 1 Swiss-port # P46061 Mouse Ran GTPase-activating protein 1
Gene Symbol	RANGAP1
Gene Full Name	Ran GTPase activating protein 1
Background	This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function	GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. [UniProt]
Research Area	Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW	64 kDa
PTM	Phosphorylated occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBC9, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated with SUMO1. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBC9.

Images (1) Click the Picture to Zoom In