ARG41693
anti-RBMS3 antibody
anti-RBMS3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RBMS3 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RBMS3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to T350-P437 of Human RBMS3. |
| Conjugation | Un-conjugated |
| Alternate Names | RNA-binding motif, single-stranded-interacting protein 3 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q6XE24 Human RNA-binding motif, single-stranded-interacting protein 3 Swiss-port # Q8BWL5 Mouse RNA-binding motif, single-stranded-interacting protein 3 |
|---|---|
| Gene Symbol | RBMS3 |
| Gene Full Name | RNA binding motif, single stranded interacting protein 3 |
| Background | This gene encodes an RNA-binding protein that belongs to the c-myc gene single-strand binding protein family. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The encoded protein was isolated by virtue of its binding to an upstream element of the alpha2(I) collagen promoter. The observation that this protein localizes mostly in the cytoplasm suggests that it may be involved in a cytoplasmic function such as controlling RNA metabolism, rather than transcription. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Function | Binds poly(A) and poly(U) oligoribonucleotides. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 48 kDa |
Images (6) Click the Picture to Zoom In
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ARG41693 anti-RBMS3 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41693 anti-RBMS3 antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG41693 anti-RBMS3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41693 anti-RBMS3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41693 anti-RBMS3 antibody WB image
Western blot: 50 µg of samples under reducing conditions. U2OS, PC-3, U-87MG, A549 and HeLa whole cell lysates stained with ARG41693 anti-RBMS3 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41693 anti-RBMS3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41693 anti-RBMS3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41693 anti-RBMS3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41693 anti-RBMS3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41693 anti-RBMS3 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat heart and Mouse testis lysates stained with ARG41693 anti-RBMS3 antibody at 0.5 µg/ml dilution, overnight at 4°C.
