ARG58166
anti-RCC1 antibody
anti-RCC1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RCC1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RCC1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-240 of Human RCC1 (NP_001260.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Regulator of chromosome condensation; RCC1-I; Chromosome condensation protein 1; SNHG3-RCC1; Cell cycle regulatory protein; CHC1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MCF7 | ||||||||
| Observed Size | 48 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P18754 Human Regulator of chromosome condensation Swiss-port # Q8VE37 Mouse Regulator of chromosome condensation |
|---|---|
| Gene Symbol | RCC1 |
| Gene Full Name | regulator of chromosome condensation 1 |
| Function | Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG58166 anti-RCC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver stained with ARG58166 anti-RCC1 antibody at 1:100 dilution.
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ARG58166 anti-RCC1 antibody WB image
Western blot: 25 µg of MCF7 cell lysate stained with ARG58166 anti-RCC1 antibody at 1:1000 dilution.
